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Construction of dose-effect curves by irradiating peripheral blood mononuclear cells analyzed with the alkaline comet assay

Construcción de curvas dosis-efecto por irradiación de células mononucleares de sangre periférica analizadas con el ensayo cometa alcalino




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Construction of dose-effect curves by irradiating peripheral blood mononuclear cells analyzed with the alkaline comet assay. (2016). Informe Científico Tecnológico, 15(1), 3-8. https://ipen.metarevistas.org/index.php/ict/article/view/14

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Construction of dose-effect curves by irradiating peripheral blood mononuclear cells analyzed with the alkaline comet assay. (2016). Informe Científico Tecnológico, 15(1), 3-8. https://ipen.metarevistas.org/index.php/ict/article/view/14

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Lenin Rueda
Jaime Rosales
Kety León
Marco Espinoza

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The  suitability  of  the  alkaline  comet  assay  to  detect  levels  of  DNA  damage  in  human monocytes  and  lymphocytes  under  radiation  was  tested.  For  this  purpose,  four  dose-effect curves by irradiation of cells isolated from blood samples of 4 young, health and non-somker donors were constructed. A gamma ray source of ⁶⁰Co was used and doses of 0, 2.5, 5 and 7 Gy  were delivered  to  blood  cells.  The  results  show  good  correlation  (R2  =  0.973,  SD  = 0.009)  between  the  radiation  dose  and  DNA  damage  as  measured  by  the  parameter  Tail Intensity  (percentage  of  DNA  in  the  tail  of  the  "comet").  Furthermore,  we  observe  the y (7.59, SD = 1.5). Potential use of this technique ogical dosimetry of ionizing radiations is being evaluated


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